ABSTRACT
High polyphenol content of cocoa pod extract causing it potential to be developed as antioxidant and tyrosinaseinhibitory agent in cosmetic preparations. Phytosome system known could enhance skin penetration of phytoconstituentlike polyphenol-rich extract. The objectives of this research were to formulate phytosome containing cocoa podextract, develop phytosome complex into face serum preparation, and determine antioxidant and tyrosinase inhibitoryactivities of the extract and the formulated serum. The cocoa pod extract was developed into phytosome system bythin-layer method using soy-phosphatidylcholine. The phytosome then develop into face serum formulation usingViscolam MAC 10 as a gelling agent. The antioxidant activity was conducted by 2,2-diphenyl-1-picrylhydrazyl radicalscavenging assay and tyrosinase inhibitory activity was conducted by colorimetric enzymatic assay. The cocoa podextract has very strong antioxidant activity with inhibitory concentration (IC50) of 17.21 ppm. The extract also hastyrosinase inhibitory activity with IC50 of 199.98 ppm. The phytosome complex containing cocoa pod extract andphosphatidylcholine (1:1) has good entrapment efficiency (90.5%) with an average particle size of 672 nm. Theformulated face serum has good physical characteristic and also has antioxidant and tyrosinase inhibitory activitiesthat equal with the marketed product (Hadalabo ultimate whitening milk, Rohto, Indonesia).